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. 2016 Apr 20;3(4):160110. doi: 10.1098/rsos.160110

Table 1.

A summary of the 19 microsatellite loci, amplified in three multiplex PCR reactions, used to genotype Pacific rat Rattus exulans samples. All primer sequences are available in the Rat Genome Database [14].

locus fluorescent label multiplex number product size range (bp) no. alleles
D7Arb16 HEX 1 87–104 9
D2Rat234 HEX 1 114–121 4
D8Mgh4 6-FAM 1 114–156 9
D12Rat36 HEX 1 182–195 8
D19Rat75 6-FAM 1 195–207 6
D10Rat20 6-FAM 2 97–128 12
D9Mit3 HEX 2 98–109 6
D11Rat7 HEX 2 131–149 5
D6Rat99 6-FAM 2 133–158 10
D5Rat83 6-FAM 2 166–180 6
D7Rat13 HEX 2 201–209 3
D15Rat77 6-FAM 2 240–261 11
D2Rat312 HEX 3 94–112 9
D17Mgh1 6-FAM 3 112–136 13
D1Rat313 6-FAM 3 153–167 8
D4Rat106 HEX 3 155–178 7
D6Rat100 6-FAM 3 174–186 7
D8Rat162 HEX 3 202–215 6
D14Rat39 6-FAM 3 223–257 14