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. 2016 Apr 1;129(7):1477–1489. doi: 10.1242/jcs.178608

Fig. 5.

Fig. 5.

A miR-184 sponge blocks the Gliotactin downregulation. apterous-GAL4-driven expression in the wing disc. Dashed lines indicate the dorsal–ventral boundary, with the apterous side on the left. (A) Co-expression of a miR-184 construct with two copies of the miR-184 sponge (SPmiR-184×2) tagged with mCherry (blue, A) blocked the downregulation of Gliotactin (green, A′). Dlg expression was normal (A″). (B) The miR-184 sponge co-expressed with Gli::EBFP. Two copies of the miR-184 sponge (red, B″) blocked the downregulation of endogenous Gliotactin (green, B′). (C) Co-expression of two copies of a bantam sponge with Gli::EBFP (red, C″). Endogenous Gliotactin (green, C′) was still downregulated. (D) The double miR-184 sponge significantly blocked the downregulation of endogenous Gliotactin (n=10 discs, ****P<0.0001, mean±s.d., one-way ANOVA) relative to the expression of Gli::EBFP alone, whereas the double bantam sponge (SPbant×2) did not. All image panels represent a single z slice. Scale bars: 15 μm.