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CTR1 is returned to the plasma membrane in Rab11-positive compartments. (A) Confocal images of cells treated with 30 μM Cu for 30 min and then Cu removed for 5 min show that endocytosed CTR1 is co-localized in small vesicles with Rab11(arrows) after Cu removal. (B) Compartments containing co-localized Rab11 and CTR1 (arrows) are dispersed near the surface membrane at 5 min post-Cu-removal. Scale bars: 5 μm in A, 2.5 μm in B.
