Fig. 5.

atl² and Rtnl1¹ decrease evoked neurotransmitter release. Average EJP traces for wild-type (w¹¹¹⁸) (A) and atl² (B) larvae at 0.4, 0.6, 1.0, and 1.5 mM Ca²⁺. In ascending [Ca²⁺], for w¹¹¹⁸ n=7, 17, 7, and 7 and for atl² n=7, 23, 7, and 7. (C) Mean±s.e.m. EJP amplitude for w¹¹¹⁸, atl², Rtnl1¹, and Rtnl1¹; atl². P-values shown represent a one-way ANOVA using a Fisher's LSD post hoc test performed with KaleidaGraph. (D) Mean±s.e.m. EJP amplitude for atl²; arm>UAS-atl⁺, Rtnl1¹; arm>UAS-Rtnl1⁺, and their respective controls using the ubiquitous arm-Gal4 driver. P-values shown represent an unpaired Student's t-test performed with KaleidaGraph. Recordings for C and D were performed at 0.6 mM Ca²⁺. (E) Mean±s.e.m. corrected quantal content for w¹¹¹⁸, Rtnl1¹, atl², and Rtnl1¹; atl². In ascending [Ca²⁺], w¹¹¹⁸ n=7, 17, 7, and 7; atl² n=7, 23, 7, and 7; Rtnl1¹ n=7, 10, 7, and 7; Rtnl1¹; atl² n=7, 7, 7, and 7. Resting membrane potentials (RMPs) were not significantly different among the four genotypes at bath [Ca²⁺] of 0.4 mM or 1.5 mM. At bath [Ca²⁺] of 0.6 mM or 1.0 mM, RMPs of wild-type and atl² were significantly different (P=0.0266, wild-type more hyperpolarized, at 0.6 mM [Ca²⁺], and P=0.0316, atl² more hyperpolarized, at 1.0 mM [Ca²⁺]). RMP of Rtnl1¹; atl² was significantly different from other genotypes at a bath [Ca²⁺] of 1.0 mM, Rtnl1¹; atl² more hyperpolarized. (F) Mean±s.e.m. corrected quantal content for elav>dcr, elav>dcr, atl^RNAi, and elav>dcr, Rtnl1^RNAi at 1.5 mM [Ca²⁺]. (G) Mean±s.e.m. mEJP amplitudes for w¹¹¹⁸, atl², Rtnl1¹, and Rtnl1¹; atl², pooled from all four [Ca²⁺]. (H) Mean±s.e.m. mEJP amplitudes for the indicated genotypes collected at 1.5 mM bath [Ca²⁺]. P-values in D,F,G represent an unpaired Student's t-test performed with KaleidaGraph. Recordings were performed in HL3 from muscle 6 in segment A6. Each scattergram data point is the average of the first 20 EJP responses (successes and failures) recorded for each larva.