Fig. 7.

Rtnl1 affects neurotransmitter release from multiple tissues at the NMJ. (A) Mean±s.e.m. EJP amplitude and corresponding percentage success for wild-type (w¹¹¹⁸), Rtnl1¹, and UAS-Rtnl1^RNAi expression in neurons, muscles and glia using elav-Gal4, Mef2-Gal4 and Gli-Gal4, respectively. (B) Mean±s.e.m. EJP amplitude and corresponding percentage success for w¹¹¹⁸, Rtnl1¹ and expression of UAS-Rtnl1⁺ in Rtnl1¹ mutants. UAS-Rtnl1⁺ was expressed ubiquitously, in motor neurons and in muscles using da-Gal4, D42-Gal4 and Mef2-Gal4, respectively. OK371, Mef2 and Gli-Gal4 denote concurrent expression of UAS-Rtnl1⁺ from motor neurons, muscles and glia. Recordings for A and B were made in HL3.1 at 0.1 mM Ca²⁺ from muscle 6 in segment A6. Each scattergram data point is the average of the first 20 EJP responses (successes and failures) recorded for each larva. P-values shown represent an unpaired Student's t-test performed with KaleidaGraph.