| Number of cycles | Denature | Anneal | Extend |
|---|---|---|---|
| 1 | 98°C, 3 min | - | - |
| 10 | 98°C, 10 s | 72°C, ramp down to 62°C at 1°C/cycle, 15 s |
72°C, 30 s |
| 25 | 98°C, 10 s | 62°C, 15 s | 72°C, 30 s |
| 1 | - | - | 72°C, 3 min |
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