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. Author manuscript; available in PMC: 2016 May 2.
Published in final edited form as: Nat Med. 2015 Jul 20;21(8):938–945. doi: 10.1038/nm.3909

Figure 4. Ratio of infiltrating PMNs to plasma cells is prognostic in diverse solid tumors.

Figure 4

(a) Prognostic associations between inferred PMN and plasma cell (PC) frequencies are significantly inversely correlated across the cancer landscape (Pearson R = −0.46, P = 0.02). Each point represents an individual cancer: triangles, blood cancers; squares, brain cancers; circles, remaining cancers. (b) Meta-z scores depict the prognostic significance of combining PMN and PC levels into a ratiometric index, for diverse solid tumors (source data are provided in Supplementary Table 6). (c) Comparison between CIBERSORT and tissue microarray analysis for PC, B-cell, and PMN frequencies in lung adenocarcinoma, using IGKC, CD20, and MPO, respectively, as surrogate markers for TMA (n = 187 specimens). Lung adenocarcinoma arrays from publicly available datasets (GSE7670 and GSE10072) were analyzed with CIBERSORT (n = 85 tumors). (d,e) Kaplan-Meier Plots depict patients stratified by (d) the median level of PMN to PC fractions inferred in lung adenocarcinoma microarray studies (P = 0.0005, log-rank test; n = 453 high and 453 low patients; Supplementary Table 6) and (e) the median level of MPO/IGKC stained positive in lung adenocarcinoma tissue sections (P = 0.028, log-rank test; n = 94 high and 93 low patients). Hazard ratios were 1.5 (1.2–1.9, 95% CI) for d and 1.7 (1.1–2.6, 95% CI) for e. Inferred PMN to PC levels were also significantly prognostic in continuous models assessed by univariate Cox regression in d (P = 0.003, Z = 2.98) and e (P = 0.0005, Z = 3.46). Data in c are presented as means ± s.e.m. All patients were right censored after 5 years in d and e.