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. Author manuscript; available in PMC: 2016 May 2.
Published in final edited form as: Brain Res Dev Brain Res. 1991 May 20;60(1):1–7. doi: 10.1016/0165-3806(91)90149-d

Figure 1.

Figure 1

The effects of decreasing dosages of morphine (10−6 M, 10−8 M, 10−10 M) on the total numbers of cells per culture dish from 3 to 8 days in vitro (DIV). Baseline cell counts for all cultures prior to treatment are indicated at day 1. Morphine treatment caused an overall decrease in cell number over this time period (ANOVA; df = 3, 80; F = 18.16; P < 0.001). Newman-Keuls test revealed no difference from control values after 3 DIV; however, at 6 DIV, all three concentrations were significantly different from control (P < 0.05) but not from each other. At 8 DIV, morphine (10−6, 10−8, or 10−10 M) caused a marked reduction in cell number compared to control values (P < 0.05), although 10−10 M morphine treatment did not reduce cell numbers as much as 10−6 or 10−8 M dosages (P < 0.05).