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. Author manuscript; available in PMC: 2017 Mar 15.
Published in final edited form as: Immunity. 2016 Mar 2;44(3):597–608. doi: 10.1016/j.immuni.2016.02.004

Figure 6. Chitosan Promotes cGAS-STING-IFNAR1-Dependent Th1 Responses and IgG2c Production.

Figure 6

(A) H1-specific intracellular IFN-γ production in CD3+CD4+ isolated from PECs of WT (white, PBS; gray, antigen alone; black, antigen+adjuvant), Ifnar1−/− (green), Tmem173−/− (red), and Mb21d1−/− (blue) mice immunized with H1 alone or in combination with chitosan.

(B) Representative dot plots showing levels of H1 specific intracellular IFN-γ in CD3+CD4+ (upper panel). Gating strategy used for analysis of intracellular IFN-γ production (lower panel).

(C) Antigen-specific IFN-γ production in mLNs isolated from WT, Ifnar1−/−, Tmem173−/−, and Mb21d1−/− mice immunized with H1 alone or with chitosan.

(D) H1-specific IgG2c in serum of WT, Ifnar1−/−, Tmem173−/−, and Mb21d1−/− mice immunized with H1 alone or with chitosan. Representative of two independent experiments for WT, Ifnar1−/−, Tmem173−/−, and one experiment for WT, Ifnar1−/−, Tmem173−/−, and Mb21d1−/−, n = 5 per group. Data shown as mean ± SEM. WT versus Ifnar1−/−, WT versus Tmem173−/−, WT versus Mb21d1−/−,*p < 0.05, ***p < 0.001. See also Figure S7.