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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Nature. 2016 Feb 8;530(7590):293–297. doi: 10.1038/nature16964

Extended Data Figure 5. Effect of NMB on rhythmic activity of preBötC slice.

Extended Data Figure 5

a, Neuronal activity trace (∫XII, black; ∫preBötC population activity, grey) of preBötC slice containing 30 nM NMB, as in Figure 2e. Note the extreme effect of NMB in which every burst (“breath”) in the trace is a doublet (“sigh”, *). Bar, 5 s. b,c, NMB increases the doublet rate by increasing the fraction of total events that are doublets (b) and decreasing the interval following a doublet (c). *, p<0.05, n=7. d,e, GRP also increases the doublet rate by increasing the fraction of total events that are doublets (d) and decreasing the interval following a doublet (e). *, p<0.05, n=9. Note that post-doublet intervals are significantly longer than post-burst intervals under all conditions, consistent with longer post-sigh apneas in vivo.