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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Nature. 2016 Feb 8;530(7590):293–297. doi: 10.1038/nature16964

Extended Data Figure 8. Expression of Grp in rodent brain.

Extended Data Figure 8

a,b, In situ hybridization of mouse brain slices as in Figure 3a showing expression of Grp (purple) in parabrachial nucleus (PBN) (a) and nucleus tractus solitarius (NTS) (b). Bar, 200 μm. c-e, In situ hybridization of rat brain slices showing expression of Grp in PBN (c), NTS (d), RTN/pFRG (e). Bar, 200 μm. f, Tiled image showing GRP-positive projection (red) from RTN/pFRG region to preBötC region containing SST-positive neuron (green). Bar, 20 μm. g-i, Serial confocal optical sections (0.8 μm apart) through mouse preBötC stained for GRP (red) and SST (green) focusing on short segment of GRP-positive projection where a GRP puncta (red) directly abuts (arrowhead) an SST-positive neuron. Bar, 10 μm.