Table 3. Positive skin test responses in a guinea pig potency test of 24 MAP recombinant proteins from 47 total proteins tested.

Protein Description	Locus Tag	Area Measurement (mm2)	Standard Deviationa
Acyl carrier protein	MAP_1997	231.52	87.70
Elongation factor Tu	MAP_4143	209.08	124.13
Electron transfer flavoprotein (Beta-subunit) FixA	MAP_3061c	192.36	87.68
Putative uncharacterized protein	MAP_2694	171.72	56.83
Putative acyl-CoA dehydrogenase	MAP_3651c	166.53	77.51
LprG protein	MAP_1138c	161.79	50.09
Major membrane protein 1	MAP_2121c	128.90	11.11
Peroxisomal multifunctional enzyme type 2	MAP_3567	242.51	N/A
Chaperone protein DnaK	MAP_3840	221.81	N/A
Putative uncharacterized protein	MAP_3692c	148.58	N/A
Lipoprotein LprC	MAP_2497c	141.95	N/A
Superoxide dismutase (Fragment)	MAP_0187c	131.90	N/A
Fructose-bisphosphate aldolase class-I	MAP_4308c	122.33	N/A
LppZ protein	MAP_3041	104.28	N/A
DNA-binding protein HU	MAP_3024c	68.78	N/A
Peptidyl-prolyl cis-trans isomerase	MAP_1693c	48.52	N/A
Fatty acid desaturase	MAP_2698c	44.25	N/A
Antigen 85-B	MAP_1609c	41.96	N/A
ATP synthase subunit alpha	MAP_2453c	41.49	N/A
Serine/threonine protein kinase	MAP_0016c	38.83	N/A
Wag31 protein	MAP_1889c	36.72	N/A
Putative uncharacterized protein	MAP_2020	29.59	N/A
Peroxiredoxin	MAP_1589c	26.12	N/A
6-phosphogluconolactonase	MAP_1174c	16.32	N/A
MBP/Lac-Z	----	0.00	N/A
PPD 9801	----	149.14	10.82
PPD 0902	----	241.84	37.60

^a For the proteins which resulted in skin test responses for both inoculation sites the values represent the average response (mm²) and the standard deviation from two guinea pigs.

For the 17 proteins, which exhibited only one positive skin test reaction the value represents the response from the single reactive inoculation and the standard deviation is not available (N/A).