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The PCR amplification results for the Francisella species using tul4 primers. Lane 1 is positive control strain F. tularensis subsp. holarctica LVS (NCTC 10857), lane 2 is negative control (water), lanes 3-8 are clinical Francisella strains. Molecular sizes in base pair (bp) are indicated at the right. (M: Molecular mass standard). This PCR assay was used to amplify a 428 bp fragment which is common in Francisella tularensis.
