Skip to main content
. 2016 Jan 8;10(1-2):66–76. doi: 10.1080/19336918.2015.1108510

Figure 2.

Figure 2.

Slit2 was expressed by human placental multipotent mesenchymal stromal cells (hPMSCs) and the Slit2 effect on HUVEC migration. (A) The Slit2 levels in the hPMSC-conditioned medium before and after Slit2 depletion by immunoprecipitation was assessed by Western blot (upper panel) and enzyme-linked immunosorbent assay (lower panel). CTL: hPMSC-conditioned medium without Slit2 depletion. Data are mean ± SD of 3 independent experiments. (B) The HUVEC migration ability was significantly reduced by EGM with 2% FBS (CTL) containing 1 μg/ml Slit2 (Slit2), or CTL mixed with a hPMSC-conditioned medium containing 2% FBS (1:1 ratio; CM), but the inhibition was abrogated by Slit2 depletion from the conditioned medium (CTL mixed with hPMSC-conditioned medium with 2% FBS after Slit2 depletion, 1:1 ratio; CM-Slit2 depletion). CTL: EGM with 2% FBS. Data are mean ± SD of 3 independent experiments.