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. 2016 Jan 8;10(1-2):66–76. doi: 10.1080/19336918.2015.1108510

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Figure 5. — Slit2 modulates the expression and interaction of Robo1 and Robo4 receptors in human umbilical vein endothelial cells (HUVECs). (A) Robo1 and Robo4 were expressed in HUVECs. The Robo1 and Robo4 levels decreased as Slit2 treatment concentration increased as shown in Western blot. (B) The interaction between Robo1 and Robo4 with or without 1 μg/mL Slit2 for 5 to 15 minutes was examined by co-immunoprecipitation (IP) in HUVEC lysate. The HUVEC Robo1 was immunoprecipitated from HUVEC lysate and probed by an antibody against Robo1, Robo4 or Slit2. Western blot (WB) revealed that endogenous Robo1 co-immunoprecipitates with Robo4. Slit2 interacted with the Robo1/4 receptor complex. The lower panel showed the input of Slit2, Robo1, Robo4 and α-tubulin.