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Methods for the isolation and molecular characterization of BCTCs.
Abbreviations: Ab, antibody; BCTC, breast cancer circulating tumor cell; CTC, circulating tumor cell; EPISPOT, epithelial immuno SPOT; FACS, fluorescence activated cell sorting; IF, immunofluorescence; ISET, isolation by size of epithelial tumor cells, FISH, fluorescence in situ hybridization; PBMC, peripheral blood mononuclear cell; PCR, polymerase chain reaction.
