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. Author manuscript; available in PMC: 2016 May 2.
Published in final edited form as: J Neurotrauma. 2012 Feb 29;29(8):1626–1637. doi: 10.1089/neu.2011.2190

FIG. 4.

FIG. 4

The anti-α4 treatment decreases neutrophils and macrophages in the kidney at 24 h after spinal cord injury (SCI). (A) Photomicrographs of kidney sections from uninjured and T4 SCI rats, immunostained by the anti-neutrophil antibody (A1–A3), and by the ED-1 antibody (A4–A6). Insets in A2 and A6 show high-power detail of cells with morphology typical of neutrophils and macrophages, respectively (g, glomerulus; t, tubule). Arrows in A2 point to neutrophils in the glomerulus and near a tubule. Arrow in A5 points to a macrophage near a tubule (scale bar = 100 μm in A6 also applies to A1–A6; scale bar = 10 μm in insets). (B) Neutrophil protein expression was examined by Western blotting in uninjured and T4 SCI rats (n = 4 for all groups). (C) Macrophage protein (ED-1) expression was also detected by Western blotting in these rats (n = 4 per group; *significantly different from uninjured; #significantly different from T4 SCI controls; U, uninjured rats; T4C, T4 control SCI rats; T4T, T4 SCI rats treated with the anti-α4 monoclonal antibody [mAb]; A.U., arbitrary units).