Biotinylated mouse anti-Ly-6G (1:800; eBiosciences, San Diego, CA) |
Ly-6G |
ExtrAvidin®-Peroxidase (1:1000; Sigma-Aldrich, St. Louis, MO), followed by diaminobenzidine (DAB; Zymed, Carlsbad, CA) |
3, 14, 42 days post-injury |
Rat mac-1 (CD11b, 1:600; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) |
CD11b |
Biotinylated goat anti-rat IgG (1:200; Jackson ImmunoResearch, West Grove, PA), followed by avidin-peroxidase conjugate (Vector Laboratories, Burlingame, CA), and diaminobenzidine (DAB; Zymed) |
3, 14, 42 days post-injury |
Rabbit anti-human myeloperoxidase (1:500; Dako, Glostrup, Denmark) |
MPO |
Biotinylated donkey anti-rabbit IgG (1:500; Jackson ImmunoResearch, West Grove, PA), followed by Avidin-peroxidase conjugate (Vector Laboratories) and diaminobenzine (DAB, Zymed) |
3, 14 days post-injury |
Rabbit anti-5-HT (1:400; ImmunoStar, Hudson, WI) |
|
Goat anti-rabbit IgG (AF488, 1:300; Invitrogen) |
42 days post-injury |