Skip to main content
. Author manuscript; available in PMC: 2016 May 2.
Published in final edited form as: J Neurotrauma. 2011 Dec 20;29(3):539–550. doi: 10.1089/neu.2011.1976

Table 1.

List of Antibodies Used in Immunohistochemistry

Primary antibody Antigen Secondary antibody Time point analyzed
Biotinylated mouse anti-Ly-6G (1:800; eBiosciences, San Diego, CA) Ly-6G ExtrAvidin®-Peroxidase (1:1000; Sigma-Aldrich, St. Louis, MO), followed by diaminobenzidine (DAB; Zymed, Carlsbad, CA) 3, 14, 42 days post-injury
Rat mac-1 (CD11b, 1:600; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA) CD11b Biotinylated goat anti-rat IgG (1:200; Jackson ImmunoResearch, West Grove, PA), followed by avidin-peroxidase conjugate (Vector Laboratories, Burlingame, CA), and diaminobenzidine (DAB; Zymed) 3, 14, 42 days post-injury
Rabbit anti-human myeloperoxidase (1:500; Dako, Glostrup, Denmark) MPO Biotinylated donkey anti-rabbit IgG (1:500; Jackson ImmunoResearch, West Grove, PA), followed by Avidin-peroxidase conjugate (Vector Laboratories) and diaminobenzine (DAB, Zymed) 3, 14 days post-injury
Rabbit anti-5-HT (1:400; ImmunoStar, Hudson, WI) Goat anti-rabbit IgG (AF488, 1:300; Invitrogen) 42 days post-injury