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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mol Neurobiol. 2015 Nov 2;53(9):6018–6032. doi: 10.1007/s12035-015-9508-4

FIGURE 2. MACF1 deletion effects in early and later stages of dendritic growth.

FIGURE 2

(A) MACF1 regulates initial dendritic growth. Cortical neurons from control (MACF1loxP/+) or MACF1loxP/loxP brains at E14.5 were cultured and transfected with Dcx-cre-iGFP at 0 day in vitro (DIV). Dendritic morphology was examined in MAP2/GFP-positive neurons at 3 DIV. Bar, 25 μm. (B) The numbers and lengths of primary and total dendrites were quantified. (C) Effect of MACF1 in later-stage dendritic branching. E14.5 control and MACF1loxP/loxP cortical neurons were cultured and transfected with Dcx-cre-iGFP at 6 DIV. MAP2/GFP-positive neurons were assessed at 12 DIV. Bar, 25 μm. (D) The numbers of dendritic branches were quantified. n = 60 cells from 3 independent cultures using 3 mice for each condition. Statistical significance was determined by two-tailed Student's t-test. ***p < 0.001.