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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mol Neurobiol. 2015 Nov 2;53(9):6018–6032. doi: 10.1007/s12035-015-9508-4

FIGURE 3. MACF1 is required for dendritic arborization of developing hippocampal neurons.

FIGURE 3

(A) Neuron-specific deletion of MACF1 leads to abnormal dendritic development in the hippocampus. Control (MACF1loxP/+) or MACF1loxP/loxP embryos at E14.5 were electroporated in utero with Dcx-cre-iGFP to target hippocampal pyramidal neurons. The electroporated mice were sacrificed at P10 and the brain samples were collected. GFP-positive neurons were assessed in the hippocampus. MACF1 deletion decreased the lengths of apical dendrites of hippocampal neurons. Scale bar: 25 μm. (B) Representative single cell traces of soma and dendrites shown in (A). (C) The length and the number of dendrites were quantified. n = 70 cells from 5 mice for each condition. Statistical significance was determined by two-tailed Student's t-test. ***p < 0.001.