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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Mol Neurobiol. 2015 Nov 2;53(9):6018–6032. doi: 10.1007/s12035-015-9508-4

FIGURE 4. Role of MACF1 in dendritic spine formation.

FIGURE 4

(A) MACF1-deleted neurons show aberrant dendritic spines. Cortical neurons from E14.5 control (MACF1loxP/+) and MACF1loxP/loxP brains were cultured for 5 days, and then transfected with a plasmid encoding Dcx-cre-iGFP. Spines were assessed in GFP-positive neurons at 14 DIV. Bar, 10 μm. (B) Higher magnification images shown in (A). (C) The numbers, lengths, and sizes of spines were quantified. n = 45 cells from 3 independent cultures using 3 mice for each condition. Statistical significance was determined by two-tailed Student's t-test. **p < 0.01. ***p < 0.001.