Table 4.
Statistics of GPCR expression levels by construct
| Construct(s) | % Surface expression1 | Surface density2 | % Total expression3 | Total density4 |
|---|---|---|---|---|
| Nt_BRIL construct | ||||
| Range | 1.85–97.00 | 18.51–481.97 | 1.25–98.75 | 18.82–1812.69 |
| Mean | 48.12 | 100.83 | 75.94 | 306.91 |
| Median | 46.63 | 85.64 | 86.95 | 210.55 |
| 25th percentile | 32.95 | 62.73 | 58.26 | 86.12 |
| 75th percentile | 62.85 | 124.80 | 94.45 | 480.28 |
| ICL3_BRIL construct | ||||
| Range | 3.45–96.65 | 11.77–865.22 | 0.50–98.50 | 13.80–1478.02 |
| Mean | 41.29 | 47.44 | 75.74 | 209.27 |
| Median | 38.35 | 33.91 | 84.05 | 88.51 |
| 25th percentile | 21.59 | 24.13 | 63.51 | 37.85 |
| 75th percentile | 56.58 | 50.45 | 92.74 | 306.69 |
1Surface expression: The ratio of all the cells expressing the target receptors on the membrane to total cells
2Surface density: The ratio of all the fluorescence of membrane proteins to total cells
3Total expression: The ratio of cells expressing the target receptors to total cells
4Total density: The ratio of all the fluorescence to total cells