Table 5.
Median expression levels by family and construct1
| Construct(s) | % Surface expression2 | Surface density3 | % Total expression4 | Total density5 |
|---|---|---|---|---|
| Rhodopsin | ||||
| Nt_BRIL | 44.35 | 84.62 | 85.45 | 179.45 |
| ICL3_BRIL | 37.15 | 33.01 | 83.10 | 73.38 |
| Secretin | ||||
| Nt_BRIL | 62.28 | 86.23 | 85.30 | 200.18 |
| ICL3_BRIL | 75.43 | 63.79 | 93.13 | 371.82 |
| Glutamate | ||||
| Nt_BRIL | 53.03 | 124.81 | 84.00 | 413.80 |
| ICL3_BRIL | 42.83 | 53.27 | 91.70 | 700.13 |
| Frizzled/Taste 2 | ||||
| Nt_BRIL | 62.18 | 76.18 | 91.28 | 327.45 |
| ICL3_BRIL | 41.13 | 35.99 | 77.63 | 151.70 |
| Adhesion | ||||
| Nt_BRIL | 62.15 | 99.97 | 91.90 | 476.01 |
| ICL3_BRIL | 44.65 | 42.38 | 86.30 | 250.56 |
1Values are given as medians from the Rhodopsin, Secretin, Glutamate, Frizzled/Taste2, and Adhesion families with 719, 16, 22, 36, and 33 members, respectively
2Surface expression: The ratio of all the cells expressing the target receptors on the membrane to total cells
3Surface density: The ratio of all the fluorescence of membrane proteins to total cells
4Total expression: The ratio of cells expressing the target receptors to total cells
5Total density: The ratio of all the fluorescence to total cells