Figure 6.

c-Jun regulates TGFβ expression in IKKβ-null cells. The wild type cells with or without BSO treatment, and the Ikkβ ^−/− cells with or without Ad-IKKβ infection or NAC treatment were examined for (A) luciferase activity following AP-1-luc plasmid transfection, and (B and C) ChIP assays for (B) c-Jun binding of the Tgfβ2 enhancer and (C) H3K4me3 modification of the Tgfβ2 promoters. The Ikkβ ^−/− cells were transfected with a dominant negative c-Jun (bdm-c-Jun) expression plasmids, and (D) together with Tgfb2-luc and examined for the luciferase activities, and (E) examined for the Tgfb2 mRNA. (F) The mRNA for Tgfb2 and Hmox-1 was examined in c-Jun ^F/F cells infected with Ad-Cre or Ad-GFP and treated with BSO. Results represent the mean values ± SD from at least three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 were considered significantly different from the un-treated wild type samples; ###P < 0.001 was significantly different from the un-treated Ikkβ ^−/− samples