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. 2016 Apr 18;9(4):497–504. doi: 10.18240/ijo.2016.04.04

Figure 3. Plasma membrane permeability elevation and PS externalization of Tetracaine-treated HCEP cells.

Figure 3

A: AO/EB double fluorescent staining. The apoptotic ratio was caculated as percentage (mean±SD) of the total number of cells based on the permeability elevation of plasma membrane of HCEP cells (n=3). B: FCM with annexin V/propidium iodide (PI) staining. The number of annexin V-positive (PS-externalized) HCEP cells exposed to 0.3125 g/L Tetracaine in each group was expressed as percentage (mean±SD) of its total cell number (n=3). aP<0.05, bP<0.01 versus control.