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. 2016 May 3;3:16. doi: 10.3389/fmolb.2016.00016

Table 2.

Mating assays to test mobilization of the Ptw Dtr region.

Mobilizable plasmid Recipient strain
B. cenocepacia K56-2 (pSU1445) B. cepacia 322 (pSU1445) B. cepacia 4461 (pSU1445) E. coli β2163 (pSU1445)
pEF022 (pBBR1:Ptw oriT+ ptwA +2/3 ptwB) <2.8 × 10−8 <1.9 × 10−8 <2.4 × 0−8 <3.1 × 10−8
pEF031 (pBBR1:Ptw oriT + ptwABC) <2.2 × 10−8 4.4 × 10−5 3.1 × 10−5 <2.7 × 10−8

B. cenocepacia K56-2 was used as donor strain. Conjugation frequencies (transconjugants/donor) represent the mean of at least three independent experiments. Positive mobilization frequencies are highlighted in bold.