Table 2.
Mobilizable plasmid | Recipient strain | |||
---|---|---|---|---|
B. cenocepacia K56-2 (pSU1445) | B. cepacia 322 (pSU1445) | B. cepacia 4461 (pSU1445) | E. coli β2163 (pSU1445) | |
pEF022 (pBBR1:Ptw oriT+ ptwA +2/3 ptwB) | <2.8 × 10−8 | <1.9 × 10−8 | <2.4 × 0−8 | <3.1 × 10−8 |
pEF031 (pBBR1:Ptw oriT + ptwABC) | <2.2 × 10−8 | 4.4 × 10−5 | 3.1 × 10−5 | <2.7 × 10−8 |
B. cenocepacia K56-2 was used as donor strain. Conjugation frequencies (transconjugants/donor) represent the mean of at least three independent experiments. Positive mobilization frequencies are highlighted in bold.