Table 3.

Mobilization of plasmids carrying the Ptw-oriT by different conjugative plasmids between E. coli strains.

Plasmids in donor				Transfer frequency
Helper (TRA Rel CP)a		Mobilizable (Ptw Dtr)		Helper	Mobilizable
pKM101	IncN TraI TraD			0.21 ± 0.11
pKM101	IncN TraI TraD	pBBR1		0.19 ± 0.12	<3.9 × 10−8
pKM101	IncN TraI TraD	pEF022	Ptw-oriT+ptwAb	0.19 ± 0.11	<2.3 × 10−8
pKM101	IncN TraI TraD	pEF031	Ptw-oriT+ptwABC	0.25 ± 0.08	<2.5 × 10−8
pOX38	IncF TraI TraD			0.51 ± 0.03
pOX38	IncF TraI TraD	pBBR1		0.34 ± 0.07	<5.6 × 10−8
pOX38	IncF TraI TraD	pEF022	Ptw-oriT+ptwAb	0.36 ± 0.37	<2.2 × 10−8
pOX38	IncF TraI TraD	pEF031	Ptw-oriT+ptwABC	0.19 ± 0.02	<1.9 × 10−8
pSU2007	IncW TrwC TrwB			0.11 ± 0.08
pSU2007	IncW TrwC TrwB	pBBR1		0.20 ± 0.16	<1.3 × 10−8
pSU2007	IncW TrwC TrwB	pEF022	Ptw-oriT+ptwAb	0.12 ± 0.11	1.5 × 10−7± 1.1 × 10−7
pSU2007	IncW TrwC TrwB	pEF031	Ptw-oriT+ptwABC	0.19 ± 0.11	8.2 × 10−8± 5.0 × 10−9
pSU2007	IncW TrwC TrwB	pEF033	Ptw-oriT(700)	0.21 ± 0.06	7.9 × 10−8± 1.3 × 10−8
pSU2007	IncW TrwC TrwB	pEF034	Ptw-oriT(700) +ptwA	0.16 ± 0.15	1.3 × 10−7± 1.0 × 10−7
pSU1443	IncW TrwC			<1.5 × 10−7
pSU1443	IncW TrwC	pBBR1		<2.2 × 10−7	<3.1 × 10−8
pSU1443	IncW TrwC	pEF031	Ptw-oriT+ptwABC	<2.9 × 10−7	<4.5 × 10−8
pSU1445	IncW TrwB			<2.8 × 10−7
pSU1445	IncW TrwB	pBBR1		<2.5 × 10−7	<2.8 × 10−8
pSU1445	IncW TrwB	pEF022	Ptw-oriT+ptwAb	<2.9 × 10−7	<3.6 × 10−8
pSU1445	IncW TrwB	pEF031	Ptw-oriT+ptwABC	<3.1 × 10−7	<1.9 × 10−8

DH5α was used as a donor and D1210 as a recipient. Transfer frequencies (transconjugants/donor) represent the mean ± SD of three independent experiments.

^aDescription of the Helper plasmid: TRA transfer system (defined by plasmid Inc group), Rel relaxase, CP coupling protein.

^bA truncated ptwB copy was also present, which was omitted for clarity. Positive mobilization frequencies are highlighted in bold.