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. 2016 Apr 27;7:11375. doi: 10.1038/ncomms11375

Figure 3. The chromatin landscape of locus 2p23.2.

Figure 3

(a) The SNP rs4407214 is included in a genomic tile overlapping chromatin features indicative of promoters and enhancers, shaded red. (b,c). Luciferase assays showing activity in the tile containing SNP rs4407214 (highlighted in pink in a.) in MCF10A and CAL51, red box plots indicate significantly different from the control tile (P<0.0001). Brown box plot indicates significant difference from the reference allele (P= 0.0059). (d) Electrophoretic mobility shift assay (EMSA) showing the formation of allele-specific complexes for rs4407214. M, major allele; m, minor allele. Lines 1, 2, 7, 8—no nuclear extract. Lines 3, 4, 5, 6—10 μg of MCF10A nuclear extract. Lines 9, 10, 11, 12—10 μg of CAL51 nuclear extract. Shift detected by comparison to bands (arrows #1 and #2).