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. 2016 Apr 26;7:11312. doi: 10.1038/ncomms11312

Figure 2. A screen of existing medications have minimal effects on rescuing OPC growth on CSPGs.

Figure 2

(a) Representative images of OPCs plated onto either BSA (control), CSPGs or CSPGs in the presence of benztropine (10 μM) or clemastine (1 μM) for 18–24 h, stained with O4. (b) Quantifications of process outgrowth of OPCs plated on CSPGs in the presence of a number of reported pro-remyelinating compounds, demonstrating a small effect with benztropine (10 μM) and clemastine (1 μM), while showing toxicity with higher concentrations of clemastine, clobetasol and miconazole (10 μM). (c) Process outgrowth of OPCs plated on CSPGs treated with a screen of 245 drugs, presented as change relative to CSPGs. None of these compounds were statistically significant for positive change compared with CSPGs, while some compounds were toxic (negative change; see Supplementary Table 3). The results are presented as four replicate wells of an individual experiment that was conducted three times (b) or once (c). *P<0.05, **P<0.01, ****P<0.0001, one-way analysis of variance with Dunnett's post hoc test (respective to CSPG control). Error bars are mean±s.d. Scale bar, 25 μm.