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. 2016 Apr 26;7:11401. doi: 10.1038/ncomms11401

Figure 4. Effect of different sterols on Lo domain formation and membrane fusion.

Figure 4

Lipid mixing with 1 μM HIV-FP added to 50 μM LUVs composed of DPPC/DOPC/DOPS/sterol (2:1:1:1; top row). Sterols from left to right are cholesterol, lanosterol, cholestenone and coprostanol as indicated. Fluorescence micrographs of supported lipid monolayers and GUVs (insets) with corresponding lipid compositions and labelled with 0.1 mol% Rh-PE (bottom row). The scale of all lipid monolayers is 60 × 60 μm2 and scale bars in insets are 10 μm. Error bars are s.d. of three replicates.