Figure 4. Conversion of CD44^lowCXCR3 ⁺ CD8 T cells to the CD44^hi phenotype. (A & B) Effect that discontinuing IL-4C treatment has on the CXCR3 ⁺ CD8 T cell population. B6 mice were treated with IL-4C for 7 days, and the percentage of total CXCR3 ⁺ , CD44^hiCXCR3 ⁺ , and CD44^lowCXCR3 ⁺ cell populations in peripheral blood CD8 T cells (A) and Eomes expression levels in CXCR3 ⁺ CD8 T cells (B) were analyzed by flow cytometry on the indicated days after treatment. Data summarized from two experiments are shown. (C & D) Conversion of CD44^lowCXCR3 ⁺ CD8 T cells to the CD44^hi phenotype by allo-stimulation. Splenic CD8 T cells from IL-4C-treated B6 mice were sorted into CD44^hiCXCR3 ⁺ and CD44^lowCXCR3 ⁺ populations by FACSAria and labeled with CTV. A total of 2×10⁵ CTV-labeled cells from each population were mixed with 3×10⁶ T cell-depleted bone marrow cells from untreated B6 mice, and transferred into irradiated BALB/c mice through intravenous injection. After the transfer, body weight was monitored every week (C). On day 14 after the cell transfer, splenocytes from the recipient BALB/c mice were labeled with antibodies against H-2K^b, CD8, CD44 and CXCR3, and the expression levels of CD44 and CXCR3 in CTV^low H-2K^b+ donor CD8 T cells that had proliferated were analyzed by flow cytometry (D). Numbers in the plots indicate the percentage of cells in each quadrant.