Fig. 1. Detection of local specific antibodies in the nasal secretion of the NPT-positive and NPT-negative groups. (A) Total IgE, (B) specific IgE to D. farinae were detected by using the ImmunoCAP, (C) specific IgE to D. farinae, (D) specific IgA to D. farinae, and (E) secretory IgA to D. farinae were measured by using ELISA. The horizontal bars indicate positive cutoff values (mean+3 SD). All values were adjusted for the protein ratio. NPT, nasal provocation test.
