Figure 2.
Gαz-null β-cells have an increased replication level independent of Ex4 treatment. A–D, Representative islet imaged from each experimental group subjected to IF analysis for insulin (red) and Ki67 (green), with a DAPI nuclear counterstain (blue). Ki67-positive β-cell nuclei are indicated by white arrows. In all 4 images, the scale bar represents 100 μm. E, Quantification of Ki67-positive β-cell nuclei calculated from all islets counted on 3 sections of each mouse pancreas separated by at least 200 μm. Data are shown as the mean ± SEM; n = 5–7 mice per group. Data for each of the 4 experimental groups was compared by two-way paired ANOVA with Dunnet's multiple comparisons test post hoc. **, P < .01 and ***, P < .001.