Skip to main content
. 2015 Feb 13;65(5):797–805. doi: 10.1136/gutjnl-2013-306464

Figure 4.

Figure 4

miR-199 precursors reverse visceral hypersensitivity (VH) via downregulation of transient receptor potential vanilloid type 1 (TRPV1). (A) Following intraperitoneal injection of microRNA (miRNA) (lenti-miR-199a precursors) into VH rats, there were significant increases in the visceral response threshold to colonic distension indicating a large reduction in VH (*p<0.01) at 8 days, but not at 3 days. (B) ELISA assay demonstrates diminished TRPV1 expression in rat colon and dorsal root ganglion (DRG) at 8 days following treatment with lenti-miR-199a precursor compared with lenti-miR-control (*p<0.05). (C and D) Fluorescence in situ hybridisation (FISH) with a labelled, specific miR-199a probe to identify key target genes that modulate VH. Increased miR-199a expression in DRG of VH rats that received lenti-miR-199a precursor treatment at 8 days (C.i) versus lenti-miR-control injections (C.iv). Decreased DRG TRPV1 expression is shown (C.ii) following lenti-miR-199a precursor treatment of VH rats compared with lenti-miR-controls (C.v). VH rats that received lenti-miR-199a precursor treatment exhibited increased miR-199a colonic expression at 8 days (D.i) versus lenti-miR-control injection (D.iv). Following lenti-miR-199a precursor treatment of VH rats, there was decreased TRPV1 expression (D.ii) compared with lenti-miR-controls (D.v). DAPI, 4′,6-diamidino-2-phenylindole.