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. 2015 Oct 5;34(2):127–135. doi: 10.1136/acupmed-2015-010840

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

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Schematic representation of the experimental protocol. One week after surgery to implant electrodes, rats received electrical dural stimulation (or no stimulation in the case of the Control group) every 2 days from day 1 to day 5 (on days 1, 3, and 5). Electroacupuncture (or sham acupuncture) pretreatment was performed daily from day 0 to day 5 (on days 0, 1, 2, 3, 4 and 5) in subgroups of rats. Behavioural analysis was carried out using HomeCageScan immediately after dural stimulation on days 1, 3, and 5. c-Fos immunofluorescence was measured in brain tissues prepared following necropsy on day 5.