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. 2015 Jun 30;75(5):916–923. doi: 10.1136/annrheumdis-2014-206996

Figure 2.

Figure 2

Endoplasmic reticulum aminopeptidase 1 (ERAP1) silencing or inhibition reduces surface human leucocyte antigen (HLA)-B27 free heavy chain (FHC) expression by HeLa.B27 or C1R.B27 cells. The expression of HLA-B27 FHCs (HC-10 antibody) and classical HLA-B27 complexes (ME-1 antibody) surface expression are compared between ctr-shRNA and ERAP1-shRNA-transduced HeLa.B27 cells (A) and C1R.B27 cells (C). Summaries are shown in (B) and (D). Neither the IgG2a (isotype control for HC-10, data shown here) nor IgG1 (isotype control for ME-1, data not shown) stains HeLa.B27 and C1R.B27 cells. Representative stains of HeLa.B27 cells (E) and C1R.B27 cells (G) treated with 1000 nM DG013 are shown. Summaries of DG013A titration (0 nM, 10 nM, 100 nM, 1000 nM) on HeLa.B27 cells and C1R.B27 cells are shown in (F) and (H). All experiments were repeated three times. Results are expressed as mean and SD. p Value was determined using unpaired two-tailed t test (*p<0.05, **p<0.01).