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. 2015 Jun 30;75(5):916–923. doi: 10.1136/annrheumdis-2014-206996

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This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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Wild type endoplasmic reticulum aminopeptidase 1 (ERAP1), but not the protective variants K528R or Q730E, increases cell surface human leucocyte antigen (HLA)-B27 free heavy chain (FHC) expression. WT-ERAP1 and protective ERAP1 variants (K528R, Q730E, K528R/Q730E) were transiently expressed in ERAP1-silenced HeLa.B27 cells and ERAAP-knockout mFib.B27 cells. (A and C) Western blot showing that ERAP1 variants have been expressed at similar levels. The expression of HLA-B27 FHCs (HC-10 antibody) and classical HLA-B27 complexes (ME-1 antibody) surface expression are compared between HeLa.B27 cells (B) and mFib.B27 cells (D) expressing different ERAP1 variants. Both experiments were repeated three times. Results are expressed as mean and SD. p Value was determined using one way ANOVA (**p<0.01, ***p<0.001).