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. 2016 May 3;7:552. doi: 10.3389/fmicb.2016.00552

FIGURE 3.

FIGURE 3

Cellulolytic activities of full length and truncated MDG proteins. Hydrolytic activities were analyzed on CMC-agar-plates (A) and in CMC-zymogram gels (B). For CMC screening plates 10 μL (5–10 μg of total protein after heat precipitation at 60°C) and for zymogram gels 20 μL (3 μg of protein after heat precipitation at 60°C (GH5 and GH5-12) or 2 μg of solubilized membrane fraction (GH5-12-12 and MDG full length)) were applied. As negative control, crude extract of E. coli BL21 (DE3) pRIL with vector pET24a without insert was used. Marker, M (B): prestained protein standard (Fermentas), GH5 (∼46 kDa) and GH5-12 (∼88 kDa), GH5-12-12 (∼125 kDa) and MDG full length (∼143 kDa).