FIGURE 4.

Substrate specificity and specific activity of the full length MDG and truncated MDG proteins. Enzyme activities were determined using the DNS assay. Substrate solutions [1% (w/v)] were incubated for 30 min with 33 μg of enriched protein fractions at 60°C. For GH5 and GH5-12 purified enzymes (after storage at -70°C) and for the full length MDG and GH5-12-12 enriched protein fractions after heat precipitation were used. microcrystalline cellulose (MCC) Avicel phosphoric acid treated (PAT) Avicel, hydroxyethyl cellulose (HEC), carboxymethyl cellulose (CMC).