FIGURE 5.

Effect of temperature (A) and pH (B) on the specific activity of the full length MDG and GH5 protein. Temperature and pH-dependent activity was determined with barley β-glucan [1% (w/v)] as substrate using the DNS assay. Substrate solutions were incubated for 30 min with 44 μg of enriched protein fractions after heat precipitation (MDG) or 28 μg of purified protein (GH5) at the indicated pH value (at 60°C) or temperature (at pH 7) in McIlvaine buffer (0.2 M Na₂HPO₄ titrated with 0.1 M citric acid).