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. 2016 May 3;7:582. doi: 10.3389/fmicb.2016.00582

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Copyright © 2016 Meale, Li, Azevedo, Derakhshani, Plaizier, Khafipour and Steele.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Similarity of the bacterial communities between weaning strategy (abrupt vs. gradual weaning; P > 0.05) and weaning status (pre- vs. post-weaning; P < 0.001). Distance between the samples, based on similarity in OTU composition (OTU similarity ≥ 97%) calculated using (A) unweighted UniFrac distances in the rumen; (B) weighted UniFrac distance in the rumen; (C) unweighted UniFrac distance in feces; and (D) weighted UniFrac distance in feces, and were visualized in principal coordinates analysis (PCoA) plots. A greater distance between two points infers a lower similarity, whereas similar OTUs cluster together. The impact of weaning and weaning strategy on the clustering pattern of microbial communities were tested using PERMANOVA (implemented in PRIMER-6 software). P < 0.05 were considered significant.