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. 2016 May 3;6:25254. doi: 10.1038/srep25254

Figure 2. Diffusion of water-soluble molecules in the agarose gel.

Figure 2

(A) Field with several immobilized GUVs (POPC with 0.5 mol% NBD-PE, green) obtained ~15 min after addition of an aliquot of SRB (red) to yield a final 2.5 μM concentration. Scale bar represents 50 μm. (B) Confocal microscopy images of an immobilized GUV (POPC with 1 mol% GM1 and 0.1 mol% DPPE-Rh, red channel) acquired after addition of CTB-Alexa (green channel) to yield a final 25 ng/mL concentration. The scale bar represents 10 μm. (C) Confocal microscopy images of two GUVs (POPC with 0.5 mol% NBD-PE, green). An aliquot of SRB (red) was added to the chamber with immobilized vesicles to yield a final 2.5 μM concentration. After establishing homogenous distribution of SRB in the chamber (6 min), an aliquot of TX-100 was added to the same sample to yield a final concentration of 1 mM (above its critical micelle concentration and therefore able to solubilize the membranes). The time stamp refers to the moment when SRB or TX-100 were added to the chamber with immobilized GUVs. The scale bar represents 20 μm.