Table 2. Generating FVII KO mice with gRNA and Cas9 mRNA co-injection.
| IDgRNAs | No. injectedembryos | No. embryostransferred | No. ofReplicates | No.recipients | No.newborns | No. of newborns containing mutant allele(s)* |
Mutant mice / total mice tested (%) (Mean ± SEM) | ||
|---|---|---|---|---|---|---|---|---|---|
| Total No.mutantmice | No. with oneallele (Alive) | No. with twoalleles (Deceased) | |||||||
| F7–1 | 78 | 71 | 3 | 3 | 18 | 1 | 1 (1) | 0 | 3.7 ± 3.7a |
| tF7–1 | 98 | 98 | 3 | 3 | 38 | 19 | 1 (1) | 18 (18) | 55.0 ± 5.0b |
| F7–2 | 110 | 106 | 3 | 4 | 24 | 8 | 2 (2) | 6 (6) | 35.8 ± 5.8a |
| tF7–2 | 75 | 67 | 3 | 3 | 20 | 15 | 15 (15) | 0 | 80.1 ± 7.4b |
| F7–3 | 80 | 77 | 3 | 3 | 6 | 2 | 2 (2) | 0 | 27.8 ± 14.7a |
| tF7–3 | 120 | 115 | 3 | 4 | 20 | 8 | 8 (8) | 0 | 39.4 ± 6.1a |
a,b values with the different numbers within the same column showed significant differences (P < 0.05). The experiment was performed with three replicates. The statistical comparison was performed in pair between std-RGNs and tru-RGNs that was F7–1 vs. tF7–1, F7–2 vs. tF7–2, F7–3 vs. tF7–3. *all newborns with one mutant allele survived, while those with double mutant alleles were survived, but deceased shortly after birth.