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. 2016 May 3;6:25283. doi: 10.1038/srep25283

Figure 3. Inhibition of DNA methylation by 5-Aza-dC at the early stage of differentiation activates Wnt/β-catenin signaling and enhances gene expression in osteoblastogenesis in 3T3-L1 cells.

Figure 3

(A) Treatment of 5-Aza-dC increases phosphorylation of GSK3β at the early stage of differentiation. (B) Treatment of 5-Aza-dC increases β-catenin content at the early stage of differentiation. (C) Treatment of 5-Aza-dC enhances the expression of osteoblastogenic markers BGLAP and Dlx5. 3T3-L1 preadipocyte were induced to differentiate with a differentiation medium as described in Materials and Methods, and were concurrently treated with either PBS or 5-Aza-dC at day 1 of differentiation (in A,B) and at the time points indicated in (C). mRNA and protein levels were measured by real-time RT-PCR and immunoblotting, respectively. Representative blots are shown in (A,B). All data are expressed as mean ± SEM (n = 3–6); *p < 0.05; **p < 0.01 vs. control.