Table 1. Human enteric glial cells secrete PUFA metabolites.
| 6kPGF1α | TxB2 | PGE3 | 11β-PGF2α | PGF2α | PGE2 | PGD2 | ||
|---|---|---|---|---|---|---|---|---|
| control | 165.19 | 674.25 | 573.32 | 386.46 | 326.92 | 252.31 | 103.01 | (pg/ml) |
| 138.45 | 619.46 | 658.93 | 198.97 | 316.03 | 222.67 | 60.68 | SD | |
| CD | 871.97 | 525.72 | 584.30 | *204.97 | 172.87 | 1181.72 | 130.69 | (pg/ml) |
| 1676.83 | 290.22 | 655.02 | 110.81 | 109.30 | 1618.89 | 80.08 | SD | |
| LxA4 | 8isoPGA2 | PGA1 | 7MaR1 | PD1 | LTB4 | 18-HEPE | ||
| control | 827.89 | 625.24 | 6.87 | 652.02 | 206.51 | 79.84 | 7176.48 | (pg/ml) |
| 520.19 | 735.16 | 24.76 | 951.28 | 252.52 | 108.10 | 8170.11 | SD | |
| CD | 803.29 | 1067.13 | 25.83 | 21.26 | 65.11 | 70.86 | *315.25 | (pg/ml) |
| 459.53 | 1086.41 | 44.59 | 39.40 | 107.30 | 81.08 | 140.46 | SD | |
| 15dPGJ2 | 15-HETE | 17-HDoHE | 14-HDoHE | 8-HETE | 12-HETE | 5-HETE | ||
| control | 535.19 | 2009.81 | 3664.70 | 4727.55 | 1099.49 | 3939.48 | 309.10 | (pg/ml) |
| 488.10 | 1662.99 | 3444.67 | 3413.61 | 987.09 | 2414.31 | 231.79 | SD | |
| CD | *46.73 | *800.78 | 1789.32 | 3377.03 | 594.34 | 2894.82 | 300.91 | (pg/ml) |
| 21.39 | 385.62 | 3163.79 | 2918.26 | 910.33 | 1675.66 | 493.48 | SD | |
| non detected | LxB4 | RvD2 | RvD1 | LTB5 | ||||
| 5,6-DiHETE | 14,15-EET | 5oxoETE | 11,12-EET | 8,9-EET | 5,6-EET |
31 polyunsaturated fatty acid (PUFA) metabolites were quantified by liquid Chromatography/tandem Mass Spectrometry in EGC supernatants. Among the 21 mediators secreted by human EGC, the 15-HETE, the 18-HEPE and two by-products of prostaglandin D2, the 15dPGJ2 and the 11βPGF2α were less present in CD EGC conditioned media as compared to control EGC conditioned media. n = 14 control EGC and n = 11 CD EGC; Mann-Whitney test; *p < 0.05 as compared to control EGC.