Figure 2. Results of the drug repositioning screen for IN dimerization inhibitors.

(A) Scatterplots of the primary screening using the developed assay. Dashed line shows the ‘hits’ selection criteria (>60% disruption of a PPI complex); z′ factor (B), SW (C)and CV (D) of each plate in the primary screening. Dashed lines show the minimum pass criteria (z′ > 0.5, S/N > 10, S/B > 3, SW > 2, CV < 20%). (E) Scatterplots of the confirmatory screening using the developed assay. Dashed line shows the ‘hits’ selection criteria (>60% disruption of a PPI complex). Z′ factor, SW and CV of the confirmatory screening were 0.99, 6.19 and 10.8% respectively. (F) Counterscreen using His₆-GST protein was used to analyze the interference between dexlansoprazole and the assay system; (G) Dexlansoprazole identified in the screening exhibited a sigmoidal dose-dependent reduction of assay signal; (H) The chemical structure of dexlansoprazole.