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| Substrate | Variant | Km (mM) | kcat (min−1) | kcat/Km (min−1 M−1) |
|---|---|---|---|---|
| (S)-8 | Y321I | 2.22 ± 0.32 | 1.21 ± 0.06 | 540 |
| M226T | 5.03 ± 0.46 | 0.20 ± 0.06 | 40 | |
| Y321IM226T | 0.43 ± 0.05 | 0.41 ± 0.02 | 930 | |
| 13 | T198I | n.d. | n.d. | n.d. |
| Y321I | 0.89 ± 0.09 | 0.35 ± 0.01 | 390 | |
| T198IY321I | 4.93 ± 0.75 | 0.68 ± 0.06 | 140 |
n.d: not determined due to negligible activity.
