Figure 3. LCB and LCFA transports into cells are competitive with each other.

(a–c) BY4741 (wild-type) cells were labeled with 20 μM [³H]DHS in the presence of cold palmitic acid (PAL) at the indicated concentration at 30 °C for 5 min (a). BY4741 cells were labeled with 20 μM [³H]palmitic acid in the presence of cold DHS at the indicated concentration at 30 °C for 30 min (b). BY4741 cells were labeled with 20 μM [³H]DHS in the presence of 20 μM cold FA as indicated (C12:0, lauric acid; C16:0, palmitic acid; C16:1, palmitoleic acid; C20:0, arachidic acid; C20:4, arachidonic acid; 2-OH C16:0, (+/−)-2-hydroxypalmitic acid) at 30 °C for 5 min (c). Radioactivities associated with cells, medium, and glass test tubes were counted by a liquid scintillation counter, and those associated with cells are expressed as a percentage of the total radioactivity. Values represent the means ± SDs of three independent experiments, and statistically significant differences are indicated (t-test; **p < 0.01).