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. 2016 May 3;6:25342. doi: 10.1038/srep25342

Figure 1. Resveratrol reversed the superoxide-dependent NO production and elevated SBP in the RVLM of rats with fructose-induced hypertension.

Figure 1

(A) The graph reveals the effects of resveratrol on SBP in the fructose groups after one week. The SBP significantly recovered after the resveratrol treatment compared with the fructose group. (B) Quantitative analysis of intracellular NO, measured by flow cytometry of DAF-2 DA florescence intensity, in control, fructose and fructose + resveratrol (n = 10,000 cells per group; One-way ANOVA). Treatment with resveratrol significantly increased the NO levels in the RVLM in the fructose group compared with the control. (C) Confocal microscopy analysis of green fluorescence was used to estimate p-AMPKT172 levels in the RVLM after treatment with resveratrol. The representative images shown demonstrate that the elevation of the AMPK phosphorylation level was significantly increased in the RVLM after treatment with resveratrol. (D) Representative images of DHE-treated brain sections. Sections of the RVLM from the fructose group showed significant increases in DHE fluorescence compared with the control group sections. Furthermore, the DHE fluorescence in the RVLM were significantly attenuated by the resveratrol treatment. (E) Confocal microscopy analysis of green fluorescence was used to estimate nitrotyrosine levels in the RVLM after treatment with resveratrol. The representative images shown demonstrate that the elevation of the nitrotyrosine level was significantly decreased in the RVLM after treatment with resveratrol. The images were photographed at ×400 magnification. We examined 3 groups (control; 10% fructose-treated; and 10% fructose+resveratrol; n = 6 for each). The values shown are the means ± SEM, n = 6. *P < 0.05, **P < 0.01. Scale bar: 20 μm.