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. 2015 Oct 1;36(5):941–953. doi: 10.1177/0271678X15606719

Figure 4.

Figure 4.

Mitochondrial function in animals subjected to fructose and TBI. Bioenergetics analysis in mitochondria isolated from hippocampal tissues of regular (con) or fructose (FRU) drinking rats, which were subjected to either sham or fluid percussion injury (FPI). (a) Oxygen consumption rate (OCR) in presence of succinate and rotenone (basal respiration) and in response to the sequential treatment with ADP, oligomycin (ATP synthase inhibitor), carbonyl cyanide-p-trifluoromethoxyphenylhydrazone (FCCP; uncoupler), and antimycin-A (complex III inhibitor) using XF24 Extracellular Flux Analyzer; (b) respiration due to ATP turnover was calculated as response to the oligomycin, while maximal mitochondrial respiratory capacity was deduced from the response to treatment with FCCP; and (c) cytochrome C oxidase activity. Data are expressed as percentage of basal OCR (mean ± SEM) of five independent experiments. #P < 0.05 versus con/sham; ANOVA (two-way) with Bonferroni’s comparisons post hoc test.